Premium
Developmental regulation of DNA replication timing at the human β globin locus
Author(s) -
Simon Itamar,
Tenzen Toyoaki,
Mostoslavsky Raul,
Fibach Eitan,
Lande Laura,
Milot Eric,
Gribnau Joost,
Grosveld Frank,
Fraser Peter,
Cedar Howard
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.21.6150
Subject(s) - biology , locus (genetics) , replication timing , genetics , dna replication , gene , control of chromosome duplication , transcription (linguistics) , microbiology and biotechnology , linguistics , philosophy
The human β globin locus replicates late in most cell types, but becomes early replicating in erythroid cells. Using FISH to map DNA replication timing around the endogenous β globin locus and by applying a genetic approach in transgenic mice, we have demonstrated that both the late and early replication states are controlled by regulatory elements within the locus control region. These results also show that the pattern of replication timing is set up by mechanisms that work independently of gene transcription.