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Regulation of replication timing in fission yeast
Author(s) -
Kim SooMi,
Huberman Joel A.
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.21.6115
Subject(s) - schizosaccharomyces pombe , replication (statistics) , biology , origin of replication , replicate , schizosaccharomyces , yeast , kinase , deoxyribonucleoside , wee1 , cell cycle , dna replication , microbiology and biotechnology , genetics , dna , saccharomyces cerevisiae , cyclin dependent kinase 1 , virology , cell , statistics , mathematics
Here we report the first characterization of replication timing and its regulation in the fission yeast Schizosaccharomyces pombe . We used three different synchronization methods: centrifugal elutriation, cdc10 temperature‐shift and release, and starvation for deoxyribonucleoside triphosphates (dNTPs) by treatment with hydroxyurea (HU) followed by removal of HU, to study the times when specific autonomously replicating sequence elements (ARS elements; potential replication origins) replicate during S phase. We found that individual ARS elements replicate at characteristic times, some early and some late, independently of synchronization method. In wild‐type cells treated with HU, early ARS elements replicated but late ones did not. However, in HU‐treated mutant cells lacking the Rad3 (similar to human ATR and ATM) or Cds1 (similar to human CHK2) checkpoint kinase, both early and late ARS elements were able to replicate. Thus under conditions of dNTP starvation the Rad3 and Cds1 kinases are needed to suppress the replication of normally late‐replicating regions.