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A specific lysine in c‐Jun is required for transcriptional repression by E1A and is acetylated by p300
Author(s) -
Vries Robert G.J.,
Prudenziati Michela,
Zwartjes Carin,
Verlaan Matty,
Kalkhoven Eric,
Zantema Alt
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.21.6095
Subject(s) - psychological repression , biology , acetylation , microbiology and biotechnology , p300 cbp transcription factors , transcription (linguistics) , c jun , transcription factor , dna binding protein , gene , biochemistry , gene expression , linguistics , philosophy , histone acetyltransferases
The adenovirus E1A protein regulates transcription of cellular genes via its interaction with the transcriptional coactivators p300/CBP. The collagenase promoter activated by the c‐Jun protein is repressed by E1A. Here we show that E1A repression is specific for c‐Jun, as E1A does not repress the collagenase promoter activated by the homologous transcription factor EB1. Using chimeras of c‐Jun and EB1, we demonstrate that a 12 amino acid region in the basic region of the c‐Jun DNA‐binding domain is essential for repression by E1A. Since repression requires the binding of p300 to E1A, we studied the involvement of p300 acetyltransferase activity in the repression mechanism. We demonstrate that c‐Jun is acetylated in vivo , and mutational analysis identified Lys271 in the c‐Jun basic region to be essential for repression of the collagenase promoter by E1A. In addition, Lys271 is acetylated both in vitro and in vivo . These results suggest that the specific repression of the collagenase promoter by E1A involves acetylation of c‐Jun.