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The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins
Author(s) -
Walther Tobias C.,
Fornerod Maarten,
Pickersgill Helen,
Goldberg Martin,
Allen Terry D.,
Mattaj Iain W.
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.20.5703
Subject(s) - nucleoporin , nuclear pore , nuclear transport , biology , nuclear lamina , microbiology and biotechnology , importin , inner membrane , nuclear protein , nuclear localization sequence , immunogold labelling , transport protein , cytoplasm , cell nucleus , genetics , transcription factor , gene , ultrastructure , anatomy , mitochondrion
The nuclear pore complex (NPC) is a large proteinaceous structure through which bidirectional transport of macromolecules across the nuclear envelope (NE) takes place. Nup153 is a peripheral NPC component that has been implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Here, Nup153 is localized by immunogold electron microscopy to a position on the nuclear ring of the NPC. Nuclear reconstitution is used to investigate the role of Nup153 in nucleo‐ cytoplasmic transport and NPC architecture. NPCs assembled in the absence of Nup153 lacked several nuclear basket components, were unevenly distributed in the NE and, unlike wild‐type NPCs, were mobile within the NE. Importin α/β‐mediated protein import into the nucleus was strongly reduced in the absence of Nup153, while transportin‐mediated import was unaffected. This was due to a reduction in import complex translocation rather than to defective receptor recycling. Our results therefore reveal functions for Nup153 in NPC assembly, in anchoring NPCs within the NE and in mediating specific nuclear import events.