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Beat‐to‐beat oscillations of mitochondrial [Ca 2+ ] in cardiac cells
Author(s) -
Robert Valérie,
Gurlini Pamela,
Tosello Valeria,
Nagai Takeharu,
Miyawaki Atsushi,
Di Lisa Fabio,
Pozzan Tullio
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.17.4998
Subject(s) - aequorin , cytosol , biology , mitochondrion , extracellular , photoprotein , chronotropic , inotrope , verapamil , calcium , myocyte , biophysics , excitatory postsynaptic potential , microbiology and biotechnology , medicine , intracellular , endocrinology , biochemistry , receptor , bioluminescence , heart rate , blood pressure , enzyme
The Ca 2+ ‐sensitive photoprotein aequorin and the new green fluorescent protein‐based fluorescent Ca 2+ indicators ‘ratiometric‐pericam’ were selectively expressed in the mitochondria, cytosol and/or nucleus of spontaneously beating ventricular myocytes from neonatal rats. This combined strategy reveals that mitochondrial [Ca 2+ ] oscillates rapidly and in synchrony with cytosolic and nuclear [Ca 2+ ]. The Ca 2+ oscillations were reduced in frequency and/or amplitude by verapamil and carbachol and were enhanced by isoproterenol and elevation of extracellular [Ca 2+ ]. An increased frequency and/or amplitude of cytosolic Ca 2+ spikes was rapidly mirrored by similar changes in mitochondrial Ca 2+ spikes and more slowly by elevations of the interspike Ca 2+ levels. The present data unequivocally demonstrate that in cardiac cells mitochondrial [Ca 2+ ] oscillates synchronously with cytosolic [Ca 2+ ] and that mitochondrial Ca 2+ handling rapidly adapts to inotropic or chronotropic inputs.