Premium
A moving DNA replication factory in Caulobacter crescentus
Author(s) -
Jensen Rasmus B.,
Wang Sherry C.,
Shapiro Lucy
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.17.4952
Subject(s) - replisome , biology , caulobacter crescentus , control of chromosome duplication , pre replication complex , dna replication , eukaryotic dna replication , origin recognition complex , microbiology and biotechnology , origin of replication , dna , semiconservative replication , genetics , cell division , chromosome , circular bacterial chromosome , cell cycle , cell , gene
The in vivo intracellular location of components of the Caulobacter replication apparatus was visualized during the cell cycle. Replisome assembly occurs at the chromosomal origin located at the stalked cell pole, coincident with the initiation of DNA replication. The replisome gradually moves to midcell as DNA replication proceeds and disassembles upon completion of DNA replication. Although the newly replicated origin regions of the chromosome are rapidly moved to opposite cell poles by an active process, the replisome appears to be an untethered replication factory that is passively displaced towards the center of the cell by the newly replicated DNA. These results are consistent with a model in which unreplicated DNA is pulled into the replication factory and newly replicated DNA is bidirectionally extruded from the complex, perhaps contributing to chromosome segregation.