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NoRC—a novel member of mammalian ISWI‐containing chromatin remodeling machines
Author(s) -
Strohner Ralf,
Nemeth Attila,
Jansa Petr,
HofmannRohrer Urs,
Santoro Raffaella,
Längst Gernot,
Grummt Ingrid
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.17.4892
Subject(s) - biology , chromatin remodeling , chromatin , transcription factor , nucleolus , general transcription factor , microbiology and biotechnology , transcription (linguistics) , nucleosome , rna polymerase ii , chromatin structure remodeling (rsc) complex , genetics , promoter , gene , gene expression , linguistics , philosophy , cytoplasm
Transcription by RNA polymerase I on nucleosomal templates requires binding of the transcription termination factor TTF‐I to a cognate site 160 bp upstream of the transcription start site. Binding of TTF‐I is accompanied by changes in the chromatin architecture which suggests that TTF‐I recruits a remodeling activity to the rDNA promoter. We have cloned a cDNA that encodes TIP5 (TTF‐I‐interacting protein 5), a 205 kDa protein that shares a number of important protein domains with WSTF (Williams syndrome transcription factor) and hAcf1/WCRF180, the largest subunits of human chromatin remodeling complexes hCHRAC and WCRF. TIP5 co‐localizes with the basal RNA polymerase I transcription factor UBF in the nucleolus and is associated with SNF2h. The cellular TIP5–SNF2h complex, termed NoRC (nucleolar remodeling complex), induces nucleosome sliding in an ATP‐ and histone H4 tail‐dependent fashion. The results suggest that NoRC is a novel nucleolar chromatin remodeling machine that may serve a role in the regulation of the rDNA locus.