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The exosome of Trypanosoma brucei
Author(s) -
Estévez Antonio M.,
Kempf Tore,
Clayton Christine
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.14.3831
Subject(s) - trypanosoma brucei , exosome , biology , exosome complex , microbiology and biotechnology , yeast , rna , genetics , microvesicles , non coding rna , gene , microrna
The yeast exosome is a complex of at least 10 essential 3′–5′ riboexonucleases which is involved in 3′‐processing of many RNA species. An exosome‐like complex has been found or predicted to exist in other eukaryotes but not in Escherichia coli . The unicellular parasite Trypanosoma brucei diverged very early in eukaryotic evolution. We show here that T.brucei contains at least eight exosome subunit homologs, but only a subset of these associate in a complex. Accordingly, the T.brucei exosome is smaller than that of yeast. Both free and complex‐associated homologs are essential for cell viability and are involved in 5.8S rRNA maturation. We suggest that the exosome was present in primitive eukaryotes, and became increasingly complex during subsequent evolution.