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An upstream repressor element plays a role in Igf2 imprinting
Author(s) -
Eden Sharon,
Constancia Miguel,
Hashimshony Tamar,
Dean Wendy,
Goldstein Beth,
Johnson Alfred C.,
Keshet Ilana,
Reik Wolf,
Cedar Howard
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.13.3518
Subject(s) - repressor , biology , psychological repression , imprinting (psychology) , genomic imprinting , trans acting , genetics , methylation , dna methylation , gene , negative regulatory element , regulation of gene expression , regulatory sequence , transcription factor , gene expression , mutant
The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele. We have identified a repressor element within this sequence and shown that repression is probably mediated through a trans ‐ acting factor, GCF2. DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo .