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Chemokine receptor homo‐ or heterodimerization activates distinct signaling pathways
Author(s) -
Mellado Mario,
RodríguezFrade José Miguel,
VilaCoro Antonio J.,
Fernández Silvia,
Martín de Ana Ana,
Jones David R.,
Torán José L.,
MartínezA Carlos
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.10.2497
Subject(s) - library science , humanities , medicine , art , computer science
Chemokine receptors of both the CC and CXC families have been demonstrated to undergo a ligand‐mediated homodimerization process required for Ca 2+ flux and chemotaxis. We show that, in the chemokine response, heterodimerization is also permitted between given receptor pairs, specifically between CCR2 and CCR5. This has functional consequences, as the CCR2 and CCR5 ligands monocyte chemotactic protein‐1 (MCP‐1) and RANTES ( r egulated upon a ctivation, n ormal T cell‐ e xpressed and s ecreted) cooperate to trigger calcium responses at concentrations 10‐ to 100‐fold lower than the threshold for either chemokine alone. Heterodimerization results in recruitment of each receptor‐associated signaling complex, but also recruits dissimilar signaling path ways such as G q/11 association, and delays activation of phosphatidyl inositol 3‐kinase. The consequences are a pertussis toxin‐resistant Ca 2+ flux and trig gering of cell adhesion rather than chemotaxis. These results show the effect of heterodimer formation on increasing the sensitivity and dynamic range of the chemokine response, and may aid in understanding the dynamics of leukocytes at limiting chemokine concentrations in vivo .