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A putative endosomal t‐SNARE links exo‐ and endocytosis in the phytopathogenic fungus Ustilago maydis
Author(s) -
WedlichSöldner Roland,
Bölker Michael,
Kahmann Regine,
Steinberg Gero
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.9.1974
Subject(s) - ustilago , biology , endosome , endocytosis , fungus , microbiology and biotechnology , fungal protein , virology , genetics , botany , gene , peptide sequence , receptor , intracellular
We identified a temperature‐sensitive mutant of the plant pathogenic fungus Ustilago maydis that is defective in the polar distribution of cell wall components and shows abnormal morphology. The affected gene, yup1 , was cloned by complementation. It encodes a putative target soluble N ‐ethylmaleimide‐sensitive fusion protein attachment protein receptor (t‐SNARE), suggesting a function in membrane fusion. A Yup1–GFP fusion protein localized to vesicles that showed rapid saltatory motion along microtubules. These vesicles are part of the endocytic pathway and accumulate at sites of active growth, thereby supporting the expansion of the hyphal tip. In yup1 ts cells, endocytosis is impaired and accumulation of Yup1‐carrying endosomes at cell poles is abolished, resulting in apolar distribution of wall components and morphological alterations. This suggests that a membrane recycling process via early endosomes supports polar growth of U.maydis .