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The role of the Sid1p kinase and Cdc14p in regulating the onset of cytokinesis in fission yeast
Author(s) -
Guertin David A.,
Chang Louise,
Irshad Farid,
Gould Kathleen L.,
McCollum Dannel
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.8.1803
Subject(s) - cytokinesis , biology , microbiology and biotechnology , mitosis , mitotic exit , schizosaccharomyces , schizosaccharomyces pombe , anaphase , spindle pole body , cyclin dependent kinase 1 , cyclin b , polo like kinase , aurora b kinase , cell cycle , genetics , cell division , spindle apparatus , cyclin , saccharomyces cerevisiae , gene , cell
Coordination of mitosis and cytokinesis is crucial for ensuring proper chromosome segregation and genomic stability. In Schizosaccharomyces pombe , the sid genes ( cdc7 , cdc11 , cdc14 , spg1 , sid1 , sid2 and sid4 ) define a signaling pathway that regulates septation and cytokinesis. Here we describe the characterization of a novel protein kinase, Sid1p. Sid1p localizes asymmetrically to one spindle pole body (SPB) in anaphase. Sid1p localization is maintained during medial ring constriction and septum synthesis and disappears prior to cell separation. Additionally, we found that Cdc14p is in a complex with Sid1p. Epistasis analysis places Sid1p–Cdc14p downstream of Spg1p–Cdc7p but upstream of Sid2p. Finally, we show that cyclin proteolysis during mitosis is unaffected by inactivating the sid pathway; in fact, loss of Cdc2–cyclin activity promotes Sid1p–Cdc14p association with the SPB, possibly providing a mechanism that couples cytokinesis with mitotic exit.