Premium
Regulation of E2F1 activity by acetylation
Author(s) -
MartínezBalbás Marian A.,
Bauer UtaMaria,
Nielsen Søren J.,
Brehm Alexander,
Kouzarides Tony
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.4.662
Subject(s) - acetylation , e2f1 , biology , histone deacetylase , histone , e2f , hdac4 , histone deacetylase 2 , transcription factor , histone h3 , microbiology and biotechnology , biochemistry , dna , gene
During the G 1 phase of the cell cycle, an E2F–RB complex represses transcription, via the recruitment of histone deacetylase activity. Phosphorylation of RB at the G 1 /S boundary generates a pool of ‘free’ E2F, which then stimulates transcription of S‐phase genes. Given that E2F1 activity is stimulated by p300/CBP acetylase and repressed by an RB‐associated deacetylase, we asked if E2F1 was subject to modification by acetylation. We show that the p300/CBP‐associated factor P/CAF, and to a lesser extent p300/CBP itself, can acetylate E2F1 in vitro and that intracellular E2F1 is acetylated. The acetylation sites lie adjacent to the E2F1 DNA‐binding domain and involve lysine residues highly conserved in E2F1, 2 and 3. Acetylation by P/CAF has three functional consequences on E2F1 activity: increased DNA‐binding ability, activation potential and protein half‐life. These results suggest that acetylation stimulates the functions of the non‐RB bound ‘free’ form of E2F1. Consistent with this, we find that the RB‐associated histone deacetylase can deacetylate E2F1. These results identify acetylation as a novel regulatory modification that stimulates E2F1's activation functions.