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Yra1p, a conserved nuclear RNA‐binding protein, interacts directly with Mex67p and is required for mRNA export
Author(s) -
Sträßer Katja,
Hurt Ed
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.3.410
Subject(s) - biology , rna , rna binding protein , messenger rna , microbiology and biotechnology , nuclear export signal , nuclear pore , biochemistry , gene , nucleus
Mex67p and Mtr2p constitute an essential mRNA export complex that interacts with poly(A) + RNA and nuclear pore proteins. We have identified Yra1p, an intranuclear protein with in vitro RNA–RNA annealing activity, which directly binds to Mex67p. The complex between Yra1p and Mex67p was reconstituted in vitro and shown by UV‐crosslinking to bind directly to RNA. Mutants of YRA1 are impaired in nuclear poly(A) + RNA export at restrictive growth conditions. ALY, the mouse homologue of Yra1p and a transcriptional co‐activator, can bind in vitro to yeast and human Mex67p and partly complements the otherwise non‐viable yra1 null mutant. Thus, Yra1p is the first RNA‐binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes.