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Immobilization of the early secretory pathway by a virus glycoprotein that binds to microtubules
Author(s) -
Xu Aimin,
Bellamy A.Richard,
Taylor John A.
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.23.6465
Subject(s) - biological sciences , library science , biology , computational biology , computer science
Membrane trafficking from the endoplasmic reticulum (ER) to the Golgi complex is mediated by pleiomorphic carrier vesicles that are driven along microtubule tracks by the action of motor proteins. Here we describe how NSP4, a rotavirus membrane glycoprotein, binds to microtubules and blocks ER‐to‐Golgi trafficking in vivo . NSP4 accumulates in a post‐ER, microtubule‐associated membrane compartment and prevents targeting of vesicular stomatitis virus glycoprotein (VSV‐G) at a pre‐Golgi step. NSP4 also redistributes β‐COP and ERGIC53, markers of a vesicular compartment that dynamically cycles between the ER and Golgi, to structures aligned along linear tracks radiating throughout the cytoplasm. This block in membrane trafficking is released when microtubules are depolymerized with nocodazole, indicating that vesicles containing NSP4 are tethered to the microtubule cytoskeleton. Disruption of microtubule‐mediated membrane transport by a viral glycoprotein may represent a novel pathogenic mechanism and provides a new experimental tool for the dissection of early steps in exocytic transport.