Premium
Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a‐cell‐specific gene
Author(s) -
Gavin Igor M.,
Kladde Michael P.,
Simpson Robert T.
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.21.5875
Subject(s) - biology , chromatin remodeling , swi/snf , chromatin , psychological repression , chia pet , regulation of gene expression , chromatin structure remodeling (rsc) complex , microbiology and biotechnology , transcription factor , gene , minichromosome , transcriptional regulation , transcription (linguistics) , scaffold/matrix attachment region , promoter , gene expression , genetics , linguistics , philosophy
In yeast, a number of regulatory proteins expressed only in specific cell types interact with general transcription factors in a combinatorial manner to control expression of cell‐type‐specific genes. We report a detailed analysis of activation and repression events that occur at the promoter of the a ‐cell‐specific STE6 gene fused to a β‐galactosidase gene in a yeast minichromosome, as well as factors that control the chromatin structure of this promoter both in the minichromosome and in the genomic STE6 locus. Mcm1p results in chromatin remodeling and is responsible for all transcriptional activity from the STE6 promoter in both wild‐type a and α cells. Matα2p cooperates with Tup1p to block both chromatin remodeling and Mcm1p‐associated activation. While Matα2p represses only Mcm1p, the Tup1p‐mediated repression involves both Mcm1p‐dependent and ‐independent mechanisms. Swi/Snf and Gcn5p, required for full induction of the STE6 gene, do not contribute to chromatin remodeling. We suggest that Tup1p can contribute to repression by blocking transcriptional activators, in addition to interacting with transcription machinery and stabilizing chromatin.