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Targeting the chromatin‐remodeling MSL complex of Drosophila to its sites of action on the X chromosome requires both acetyl transferase and ATPase activities
Author(s) -
Gu Weigang,
Wei Xierong,
Pannuti Antonio,
Lucchesi John C.
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.19.5202
Subject(s) - biology , chromatin remodeling , chromatin , bromodomain , acetylation , histone , dosage compensation , histone h4 , histone acetyltransferase , ectopic expression , genetics , multiprotein complex , microbiology and biotechnology , chromosome , dna , gene
Dosage compensation in Drosophila is mediated by a multiprotein, RNA‐containing complex that associates with the X chromosome at multiple sites. We have investigated the role that the enzymatic activities of two complex components, the histone acetyltransferase activity of MOF and the ATPase activity of MLE, may have in the targeting and association of the complex with the X chromosome. Here we report that MLE and MOF activities are necessary for complexes to access the various X chromosome sites. The role that histone H4 acetylation plays in this process is supported by our observations that MOF overexpression leads to the ectopic association of the complex with autosomal sites.