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Dynamic relocalization of phage φ29 DNA during replication and the role of the viral protein p16.7
Author(s) -
Meijer Wilfried J.J.,
Lewis Peter J.,
Errington Jeff,
Salas Margarita
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.15.4182
Subject(s) - biology , nucleoid , dna replication , control of chromosome duplication , phagemid , dna , microbiology and biotechnology , origin recognition complex , eukaryotic dna replication , bacteriophage , genetics , escherichia coli , gene
We have examined the localization of DNA replication of the Bacillus subtilis phage φ29 by immunofluorescence. To determine where phage replication was localized within infected cells, we examined the distribution of phage replication proteins and the sites of incorporation of nucleotide analogues into phage DNA. On initiation of replication, the phage DNA localized to a single focus within the cell, nearly always towards one end of the host cell nucleoid. At later stages of the infection cycle, phage replication was found to have redistributed to multiple sites around the periphery of the nucleoid, just under the cell membrane. Towards the end of the cycle, phage DNA was once again redistributed to become located within the bulk of the nucleoid. Efficient redistribution of replicating phage DNA from the initial replication site to various sites surrounding the nucleoid was found to be dependent on the phage protein p16.7.