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The chromo domain protein Chd1p from budding yeast is an ATP‐dependent chromatin‐modifying factor
Author(s) -
Tran Hien G,
Steger David J,
Iyer Vishwanath R,
Johnson Alexander D
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.10.2323
Subject(s) - biology , nucleosome , chromatin , transcription factor , swi/snf , genetics , microbiology and biotechnology , chromatin remodeling , subfamily , dna binding protein , chromatin structure remodeling (rsc) complex , dna binding domain , gene
CHD proteins are members of the chromo domain family, a class of proteins involved in transcription, DNA degradation and chromatin structure. In higher eukaryotes, there are two distinct subfamilies of CHD proteins: CHD1 and CHD3/4. Analyses carried out in vitro indicate that the CHD3/4 proteins may regulate transcription via alteration of chromatin structure. However, little is known about the role of CHD proteins in vivo , particularly the CHD1 subfamily. To understand better the cellular function of CHD proteins, we initiated a study on the Chd1p protein from budding yeast. Using genomic DNA arrays, we identified genes whose expression is affected by the absence of Chd1p. A synthetic‐lethal screen uncovered genetic interactions between SWI/SNF genes and CHD1 . Biochemical experiments using Chd1p purified from yeast showed that it reconfigures the structure of nucleosome core particles in a manner distinct from the SWI–SNF complex. Taken together, these results suggest that Chd1p functions as a nucleosome remodeling factor, and that Chd1p may share overlapping roles with the SWI–SNF complex to regulate transcription.