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The C‐terminal domain of Armadillo binds to hypophosphorylated Teashirt to modulate Wingless signalling in Drosophila
Author(s) -
Gallet Armel,
Angelats Corinne,
Erkner Alfrun,
Charroux Bernard,
Fasano Laurent,
Kerridge Stephen
Publication year - 1999
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/18.8.2208
Subject(s) - biology , armadillo , drosophila (subgenus) , terminal (telecommunication) , microbiology and biotechnology , signalling , c terminus , genetics , gene , amino acid , telecommunications , computer science
Wnt signalling is a key pathway for tissue patterning during animal development. In Drosophila , the Wnt protein Wingless acts to stabilize Armadillo inside cells where it binds to at least two DNA‐binding factors which regulate specific target genes. One Armadillo‐binding protein in Drosophila is the zinc finger protein Teashirt. Here we show that Wingless signalling promotes the phosphorylation and the nuclear accumulation of Teashirt. This process requires the binding of Teashirt to the C‐terminal end of Armadillo. Finally, we present evidence that the serine/threonine kinase Shaggy is associated with Teashirt in a complex. We discuss these results with respect to current models of Armadillo/β‐catenin action for the transmission of the Wingless/Wnt pathway.