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DNA damage‐inducible phosphorylation of p53 at N‐terminal sites including a novel site, Ser20, requires tetramerization
Author(s) -
Shieh SheauYann,
Taya Yoichi,
Prives Carol
Publication year - 1999
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/18.7.1815
Subject(s) - phosphorylation , dna damage , biology , dna , c terminus , microbiology and biotechnology , dna repair , biochemistry , amino acid
Upon DNA damage, p53 has been shown to be modified at a number of N‐terminal phosphorylation sites including Ser15 and ‐33. Here we show that phosphorylation is induced as well at a novel site, Ser20. Phosphorylation at Ser15, ‐20 and ‐33 can occur within minutes of DNA damage. Interestingly, while the DNA‐binding activities of p53 appear to be dispensable, efficient phosphorylation at these three sites requires the tetramerization domain of p53. Substitution of an artificial tetramerization domain for this region also permits phosphorylation at the N‐terminus, suggesting that oligomerization is important for DNA damage‐induced signalling to p53.