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The essential role of yeast topoisomerase III in meiosis depends on recombination
Author(s) -
Gangloff Serge,
de Massy Bernard,
Arthur Lane,
Rothstein Rodney,
Fabre Francis
Publication year - 1999
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/18.6.1701
Subject(s) - biology , homologous recombination , meiosis , genetic recombination , genetics , flp frt recombination , mutant , chromosome segregation , gene , ectopic recombination , helicase , recombination , chromosome , microbiology and biotechnology , rna
Yeast cells mutant for TOP3 , the gene encoding the evolutionary conserved type I‐5′ topoisomerase, display a wide range of phenotypes including altered cell cycle, hyper‐recombination, abnormal gene expression, poor mating, chromosome instability and absence of sporulation. In this report, an analysis of the role of TOP3 in the meiotic process indicates that top3 Δ mutants enter meiosis and complete the initial steps of recombination. However, reductional division does not occur. Deletion of the SPO11 gene, which prevents recombination between homologous chromosomes in meiosis I division, allows top3 Δ mutants to form viable spores, indicating that Top3 is required to complete recombination successfully. A topoisomerase activity is involved in this process, since expression of bacterial TopA in yeast top3 Δ mutants permits sporulation. The meiotic block is also partially suppressed by a deletion of SGS1 , a gene encoding a helicase that interacts with Top3. We propose an essential role for Top3 in the processing of molecules generated during meiotic recombination.