p53 DNA binding can be modulated by factors that alter the conformational equilibrium

The p53 tumor suppressor protein is a dimer of dimers that binds its consensus DNA sequence (containing two half‐sites) as a pair of clamps. We show here that after one wild‐type dimer of a tetramer binds to a half‐site on the DNA, the other (unbound) dimer can be in either the wild‐type or the mutant conformation. An equilibrium state between these two conformations exists and can be modulated by two types of regulators. One type modifies p53 biochemically and determines the intrinsic balance of the equilibrium. The other type of regulator binds directly to one or both dimers in a p53 tetramer, trapping each dimer in one or the other conformation. In the wild‐type conformation, the second dimer can bind to the second DNA half‐site, resulting in drastically enhanced stability of the p53–DNA complex. Importantly, a genotypically mutant p53 can also be in equilibrium with the wild‐type conformation, and when trapped in this conformation can bind DNA.