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Keratinocyte‐specific ablation of Stat3 exhibits impaired skin remodeling, but does not affect skin morphogenesis
Author(s) -
Sano Shigetoshi,
Itami Satoshi,
Takeda Kiyoshi,
Tarutani Masahito,
Yamaguchi Yuji,
Miura Hiroyuki,
Yoshikawa Kunihiko,
Akira Shizuo,
Takeda Junji
Publication year - 1999
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/18.17.4657
Subject(s) - biology , morphogenesis , microbiology and biotechnology , keratinocyte , ablation , affect (linguistics) , tissue remodeling , anatomy , immunology , medicine , genetics , gene , inflammation , cell culture , linguistics , philosophy
To elucidate the biological role of Stat3 in the skin, conditional gene targeting using the Cre‐ loxP system was performed as germline Stat3 ablation leads to embryonic lethality. K5‐Cre;Stat3 flox/− transgenic mice, whose epidermal and follicular keratinocytes lack functional Stat3, were viable and the development of epidermis and hair follicles appeared normal. However, hair cycle and wound healing processes were severely compromised. Furthermore, mutant mice expressed sparse hair and developed spontaneously occurring ulcers with age. Growth factor‐dependent in vitro migration of Stat3 ‐disrupted keratinocytes was impaired despite normal proliferative responses. We therefore conclude that Stat3 plays a crucial role in transducing a signal required for migration but not for proliferation of keratinocytes, and that Stat3 is essential for skin remodeling, including hair cycle and wound healing.