Premium
Transcriptional regulation by the γ5 subunit of a heterotrimeric G protein during adipogenesis
Author(s) -
Park JinGyoon,
Muise Aleixo,
He GongPing,
Kim SungWoo,
Ro HyoSung
Publication year - 1999
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/18.14.4004
Subject(s) - heterotrimeric g protein , biology , adipogenesis , protein subunit , microbiology and biotechnology , g protein , genetics , gene , signal transduction
The adipocyte enhancer‐binding protein (AEBP1) is a novel transcriptional repressor with carboxypeptidase activity. A two‐hybrid screen was conducted to identify components of AEBP1 that might be important in regulating its activity. The γ5 subunit of a heterotrimeric G protein was shown to bind specifically to AEBP1 and to attenuate its transcriptional repression activity. Adipogenic stimulation selectively decreased the Gγ5 level and enhanced the transcriptional repression activity of AEBP1 during mitotic clonal expansion at the onset of adipogenesis. Thus, the actions of Gγ5 and AEBP1 are directly linked, which could provide the basis for the regulation of transcription at the onset of differentiation. This report shows that a signal‐transducing molecule is involved, by direct protein–protein interaction, in the regulation of transcription during adipogenesis.