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The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding
Author(s) -
Altuvia Shoshy,
Zhang Aixia,
Argaman Liron,
Tiwari Anita,
Storz Gisela
Publication year - 1998
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/17.20.6069
Subject(s) - biology , rpos , rna polymerase , ribosome , rna , translation (biology) , untranslated region , escherichia coli , gene expression , microbiology and biotechnology , messenger rna , biochemistry , gene , promoter
OxyS is a small untranslated RNA which is induced in response to oxidative stress in Escherichia coli . This novel RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, including the fhlA ‐encoded transcriptional activator and the rpoS ‐encoded σ s subunit of RNA polymerase. Deletion analysis of OxyS showed that different domains of the small RNA are required for the regulation of fhlA and rpoS . We examined the mechanism of OxyS repression of fhlA and found that the OxyS RNA inhibits fhlA translation by pairing with a short sequence overlapping the Shine–Dalgarno sequence, thereby blocking ribosome binding/translation.