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Chromatin interaction mechanism of transcriptional control in vivo
Author(s) -
Gribnau Joost,
de Boer Ernie,
Trimborn Tolleiv,
Wijgerde Mark,
Milot Eric,
Grosveld Frank,
Fraser Peter
Publication year - 1998
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/17.20.6020
Subject(s) - erasmus+ , biology , genetics , art history , history , the renaissance
We have used a kinetic analysis to distinguish possible mechanisms of activation of transcription of the different genes in the human β globin locus. Based on in situ studies at the single‐cell level we have previously suggested a dynamic mechanism of single genes alternately interacting with the locus control region (LCR) to activate transcription. However, those steady‐state experiments did not allow a direct measurement of the dynamics of the mechanism and the presence of loci with in situ primary transcript signals from two β‐like genes in cis has left open the possibility that multiple genes in the locus could initiate transcription simultaneously. Kinetic assays involving removal of a block to transcription elongation in conjunction with RNA FISH show that multiple β gene primary transcript signals in cis represent a transition between alternating transcriptional periods of single genes, supporting a dynamic interaction mechanism.