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Host proteins can stimulate Tn 7 transposition: a novel role for the ribosomal protein L29 and the acyl carrier protein
Author(s) -
Sharpe Pamela L.,
Craig Nancy L.
Publication year - 1998
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/17.19.5822
Subject(s) - biology , ribosomal protein , transposable element , transposition (logic) , dna binding protein , genetics , dna , microbiology and biotechnology , mutant , gene , ribosome , transcription factor , rna , linguistics , philosophy
The bacterial transposon Tn 7 is distinguished by its ability to insert at a high frequency into a specific site in the Escherichia coli chromosome called attTn7 . Tn 7 insertion into attTn7 requires four Tn 7 ‐encoded transposition proteins: TnsA, TnsB, TnsC and TnsD. The selection of attTn7 is determined by TnsD, a sequence‐specific DNA‐binding protein. TnsD binds attTn7 and interacts with TnsABC, the core transposition machinery, which facilitates the insertion of Tn 7 into attTn7 . In this work, we report the identification of two host proteins, the ribosomal protein L29 and the acyl carrier protein (ACP), which together stimulate the binding of TnsD to attTn7 . The combination of L29 and ACP also stimulates Tn 7 transposition in vitro . Interestingly, mutations in L29 drastically decrease Tn 7 transposition in vivo , and this effect of L29 on Tn 7 transposition is specific for TnsABC+D reactions.