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Tad1p, a yeast tRNA‐specific adenosine deaminase, is related to the mammalian pre‐mRNA editing enzymes ADAR1 and ADAR2
Author(s) -
Gerber André,
Grosjean Henri,
Melcher Thorsten,
Keller Walter
Publication year - 1998
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/17.16.4780
Subject(s) - rna editing , biology , inosine , biochemistry , transfer rna , rna , adenosine deaminase , adar , microbiology and biotechnology , saccharomyces cerevisiae , enzyme , adenosine , gene
We have identified an RNA‐specific adenosine deaminase (termed Tad1p/scADAT1) from Saccharomyces cerevisiae that selectively converts adenosine at position 37 of eukaryotic tRNA Ala to inosine. The activity of purified recombinant Tad1p depends on the conformation of its tRNA substrate and the enzyme was found to be inactive on all other types of RNA tested. Mutant strains in which the TAD1 gene is disrupted are viable but lack Tad1p enzyme activity and their tRNA Ala is not modified at position A 37 . Transformation of the mutant cells with the TAD1 gene restored enzyme activity. Tad1p has significant sequence similarity with the mammalian editing enzymes which act on specific precursor‐mRNAs and on long double‐stranded RNA. These findings suggest an evolutionary link between pre‐mRNA editing and tRNA modification.