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Aut2p and Aut7p, two novel microtubule‐associated proteins are essential for delivery of autophagic vesicles to the vacuole
Author(s) -
Lang Thomas,
Schaeffeler Elke,
Bernreuther Daniela,
Bredschneider Monika,
Wolf Dieter H.,
Thumm Michael
Publication year - 1998
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/17.13.3597
Subject(s) - vacuole , biology , autophagy , microtubule , microbiology and biotechnology , vesicle , cytoplasm , autophagosome , saccharomyces cerevisiae , biochemistry , yeast , membrane , apoptosis
AUT2 and AUT7 , two novel genes essential for autophagocytosis in the yeast Saccharomyces cerevisiae were isolated. AUT7 was identified as a low copy suppressor of autophagic defects in aut2‐1 cells. Aut7p is a homologue of the rat microtubule‐associated protein (MAP) light chain 3 (LC3). Aut2p and Aut7p interact physically. Aut7p is attached to microtubules via Aut2p, which interacts with tubulins Tub1p and Tub2p. aut2 ‐ and aut7 ‐deleted cells are unable to deliver autophagic vesicles and the precursor of aminopeptidase I to the vacuole. Double membrane‐layered autophagosome‐like vesicles accumulate in the cytoplasm of these cells. Our findings suggest that microtubules and an attached protein complex of Aut2p and Aut7p are involved in the delivery of autophagic vesicles to the vacuole.