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RNA polymerase I transcription on nucleosomal templates: the transcription termination factor TTF‐I induces chromatin remodeling and relieves transcriptional repression
Author(s) -
Längst Gernot,
Blank Thiemo A.,
Becker Peter B.,
Grummt Ingrid
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.4.760
Subject(s) - biology , chromatin remodeling , transcription factor ii f , rna polymerase ii , transcription factor ii d , transcription (linguistics) , chromatin , termination factor , microbiology and biotechnology , general transcription factor , rna polymerase ii holoenzyme , transcription factor , chromatin structure remodeling (rsc) complex , genetics , rna polymerase , rna , transcriptional regulation , promoter , dna , gene expression , gene , linguistics , philosophy
Eukaryotic ribosomal gene promoters are preceded by a terminator element which is recognized by the transcription termination factor TTF‐I. We have studied the function of this promoter‐proximal terminator and show that binding of TTF‐I is the key event which leads to ATP‐dependent nucleosome remodeling and transcriptional activation of mouse rDNA pre‐assembled into chromatin. We have analyzed TTF‐I mutants for their ability to bind to free or nucleosomal DNA, and show that the DNA binding domain of TTF‐I on its own is not sufficient for interaction with chromatin, indicating that specific protein features exist that endow a transcription factor with chromatin binding and remodeling properties. This first analysis of RNA polymerase I transcription in chromatin provides a clue for the function of the upstream terminator and establishes a dual role for TTF‐I both as a termination factor and a chromatin‐specific transcription activator.