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Initiation and bidirectional propagation of chromatin assembly from a target site for nucleotide excision repair
Author(s) -
Gaillard PierreHenri L.,
Moggs Jonathan G.,
Roche Danièle M.J.,
Quivy JeanPierre,
Becker Peter B.,
Wood Richard D.,
Almouzni Geneviève
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.20.6281
Subject(s) - biology , nucleosome , chromatin , nucleotide excision repair , microbiology and biotechnology , dna , dna repair , genetics
To restore full genomic integrity in a eukaryotic cell, DNA repair processes have to be coordinated with the resetting of nucleosomal organization. We have established a cell‐free system using Drosophila embryo extracts to investigate the mechanism linking de novo nucleosome formation to nucleotide excision repair (NER). Closed‐circular DNA containing a uniquely placed cisplatin–DNA adduct was used to follow chromatin assembly specifically from a site of NER. Nucleosome formation was initiated from a target site for NER. The assembly of nucleosomes propagated bidirectionally, creating a regular nucleosomal array extending beyond the initiation site. Furthermore, this chromatin assembly was still effective when the repair synthesis step in the NER process was inhibited.