Premium
A novel p53‐inducible gene, PAG608 , encodes a nuclear zinc finger protein whose overexpression promotes apoptosis
Author(s) -
Israeli David,
Tessler Ellen,
Haupt Ygal,
Elkeles Adi,
Wilder Sylvia,
Amson Robert,
Telerman Adam,
Oren Moshe
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.14.4384
Subject(s) - molecular cell biology , zinc finger , library science , biology , gene , genetics , microbiology and biotechnology , computer science , transcription factor
The biological effects of the p53 tumor suppressor protein are elicited, at least in part, through sequence‐specific transactivation of a battery of target genes. The differential display method was employed towards identifying additional p53 target genes, with emphasis on genes whose induction may contribute to p53‐mediated apoptosis. We report here the cloning of a novel p53‐inducible gene, designated PAG608 . PAG608 transcripts are induced by DNA damage in a p53‐dependent manner. PAG608 encodes a nuclear zinc finger protein, which appears to localize preferentially to nucleoli when expressed at moderate levels in transfected cells. Transient overexpression of PAG608 in human tumor‐derived cells leads to distinctive changes in nuclear morphology, and can promote apoptosis. Together with additional p53 target genes, PAG608 may therefore play a role in mediating the biological activities of p53.