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Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria
Author(s) -
Rusché Laura N.,
CruzReyes Jorge,
Piller Kenneth J.,
SollnerWebb Barbara
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.13.4069
Subject(s) - biology , trypanosoma brucei , mitochondrion , enzyme , biochemistry , computational biology , microbiology and biotechnology , gene
Kinetoplastid mitochondrial RNA editing, the insertion and deletion of U residues, is catalyzed by sequential cleavage, U addition or removal, and ligation reactions and is directed by complementary guide RNAs. We have purified a ∼20S enzymatic complex from Trypanosoma brucei mitochondria that catalyzes a complete editing reaction in vitro . This complex possesses all four activities predicted to catalyze RNA editing: gRNA‐directed endonuclease, terminal uridylyl transferase, 3′ U‐specific exonuclease, and RNA ligase. However, it does not contain other putative editing complex components: gRNA‐independent endonuclease, RNA helicase, endogenous gRNAs or pre‐mRNAs, or a 25 kDa gRNA‐binding protein. The complex is composed of eight major polypeptides, three of which represent RNA ligase. These findings identify polypeptides representing catalytic editing factors, reveal the nature of this ∼20S editing complex, and suggest a new model of editosome assembly.