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Assembly of a strong promoter following IS 911 circularization and the role of circles in transposition
Author(s) -
TonHoang Bao,
Bétermier Mireille,
Polard Patrice,
Chandler Michael
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.11.3357
Subject(s) - biology , transposition (logic) , genetics , dna transposable elements , transposable element , gene , genome , philosophy , linguistics
When supplied with high levels of the IS 911 ‐encoded transposase, IS 911 ‐based transposons can excise as circles in which the right and left terminal inverted repeats are abutted. Formation of the circle junction is shown here to create a promoter, p junc , which is significantly stronger than the indigenous promoter, p IRL , and is also capable of driving expression of the IS 911 transposition proteins. High transposase expression from the circular transposon may promote use of the circle as an integration substrate. The results demonstrate that IS 911 circles are highly efficient substrates for insertion into a target molecule in vivo . Insertion leads to the disassembly of p junc and thus to a lower level of synthesis of the transposition proteins. The observation that normal levels of IS 911 transposition proteins supplied by wild‐type copies of IS 911 are also capable of generating transposon circles, albeit at a low level, reinforces the idea that the transposon circles might form part of the natural transposition cycle of IS 911 . These observations form the elements of a feedback control mechanism and have been incorporated into a model describing one possible pathway of IS 911 transposition.