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Licensing of DNA replication by a multi‐protein complex of MCM/P1 proteins in Xenopus eggs
Author(s) -
Kubota Yumiko,
Mimura Satoru,
Nishimoto Shinichi,
Masuda Taro,
Nojima Hiroshi,
Takisawa Haruhiko
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.11.3320
Subject(s) - xenopus , biology , origin recognition complex , chromatin , dna replication , control of chromosome duplication , eukaryotic dna replication , dna replication factor cdt1 , minichromosome maintenance , pre replication complex , licensing factor , microbiology and biotechnology , replication factor c , mitosis , dna , genetics , gene
In eukaryotes, chromosomal DNA is licensed for a single round of replication in each cell cycle. Xenopus MCM3 protein has been implicated in the licensing of replication in egg extract. We have cloned cDNAs encoding five immunologically distinct proteins associated with Xenopus MCM3 as members of the MCM/P1 family. Six Xenopus MCM proteins formed a physical complex in the egg extract, bound to unreplicated chromatin before the formation of nuclei, and apparently displaced from replicated chromatin. The requirement of six XMCM proteins for the replication activity of the egg extract before nuclear formation suggests that their re‐association with replicated chromatin at the end of the mitotic cell cycle is a key step for the licensing of replication.