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rqh1 + , a fission yeast gene related to the Bloom‘s and Werner's syndrome genes, is required for reversible S phase arrest
Author(s) -
Stewart Elspeth,
Chapman Carolyn Riley,
AlKhodairy Fahad,
Carr Antony M.,
Enoch Tamar
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.10.2682
Subject(s) - library science , medical school , genealogy , art history , biology , history , medicine , medical education , computer science
In eukaryotic cells, S phase can be reversibly arrested by drugs that inhibit DNA synthesis or DNA damage. Here we show that recovery from such treatments is under genetic control and is defective in fission yeast rqh1 mutants. rqh1 + , previously known as hus2 + , encodes a putative DNA helicase related to the Escherichia coli RecQ helicase, with particular homology to the gene products of the human BLM and WRN genes and the Saccharomyces cerevisiae SGS1 gene. BLM and WRN are mutated in patients with Bloom‘s syndrome and Werner's syndrome respectively. Both syndromes are associated with genomic instability and cancer susceptibility. We show that, like BLM and SGS1, rqh1 + is required to prevent recombination and that in fission yeast suppression of inappropriate recombination is essential for reversible S phase arrest.