Premium
Differential targeting of closely related ECM glycoproteins: the pherophorin family from Volvox
Author(s) -
Godl Klaus,
Hallmann Armin,
Wenzl Stephan,
Sumper Manfred
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.1.25
Subject(s) - philosophy
The alga Volvox carteri represents one of the simplest multicellular organisms. Its extracellular matrix (ECM) is modified under developmental control, e.g. under the influence of the sex‐inducing pheromone that triggers development of males and females at a concentration below 10 −16 M. A novel ECM glycoprotein (pherophorin‐S) synthesized in response to this pheromone was identified and characterized. Although being a typical member of the pherophorins, which are identified by a C‐terminal domain with sequence homology to the sex‐inducing pheromone, pherophorin‐S exhibits a completely novel set of properties. In contrast to the other members of the family, which are found as part of the insoluble ECM structures of the cellular zone, pherophorin‐S is targeted to the cell‐free interior of the spherical organism and remains in a soluble state. A main structural difference is the presence of a polyhydroxyproline spacer in pherophorin‐S that is linked to a saccharide containing a phosphodiester bridge between two arabinose residues. Sequence comparisons indicate that the self‐assembling proteins that create the main parts of the complex Volvox ECM have evolved from a common ancestral gene.