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T‐DNA integration into the Arabidopsis genome depends on sequences of pre‐insertion sites
Author(s) -
Brunaud Véronique,
Balzergue Sandrine,
Dubreucq Bertrand,
Aubourg Sébastien,
Samson Franck,
Chauvin Stéphanie,
Bechtold Nicole,
Cruaud Corinne,
DeRose Richard,
Pelletier Georges,
Lepiniec Loïc,
Caboche Michel,
Lecharny Alain
Publication year - 2002
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kvf237
Subject(s) - dna , biology , in vitro recombination , base pair , genetics , dna sequencing , arabidopsis , recombination , microbiology and biotechnology , gene , molecular cloning , complementary dna , mutant
A statistical analysis of 9000 flanking sequence tags characterizing transferred DNA (T‐DNA) transformants in Arabidopsis sheds new light on T‐DNA insertion by illegitimate recombination. T‐DNA integration is favoured in plant DNA regions with an A‐T‐rich content. The formation of a short DNA duplex between the host DNA and the left end of the T‐DNA sets the frame for the recombination. The sequence immediately downstream of the plant A‐T‐rich region is the master element for setting up the DNA duplex, and deletions into the left end of the integrated T‐DNA depend on the location of a complementary sequence on the T‐DNA. Recombination at the right end of the T‐DNA with the host DNA involves another DNA duplex, 2–3 base pairs long, that preferentially includes a G close to the right end of the T‐DNA.