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Role of the AtRad1p endonuclease in homologous recombination in plants
Author(s) -
Dubest Sandra,
Gallego Maria E.,
White Charles I.
Publication year - 2002
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kvf211
Subject(s) - homologous recombination , biology , flp frt recombination , mitotic crossover , endonuclease , mutant , saccharomyces cerevisiae , recombination , homologous chromosome , genetic recombination , non homologous end joining , dna repair , arabidopsis , genetics , dna , arabidopsis thaliana , microbiology and biotechnology , gene
Using a specific recombination assay, we show in the plant Arabidopsis thaliana that AtRad1 protein plays a role in the removal of non‐homologous tails in homologous recombination. Recombination in the presence of non‐homologous overhangs is reduced 11‐fold in the atrad1 mutant compared with the wild‐type plants. AtRad1p is the A. thaliana homologue of the human Xpf and Saccharomyces cerevisiae Rad1 proteins. Rad1p is a subunit of the Rad1p/Rad10p structure‐specific endonuclease that acts in nucleotide excision repair and inter‐strand crosslink repair. This endonuclease also plays a role in mitotic recombination to remove non‐homologous, 3′‐ended overhangs from recombination intermediates. The Arabidopsis atrad1 mutant ( uvh1 ), unlike rad1 mutants known from other eukaryotes, is hypersensitive to ionizing radiation. This last observation may indicate a more important role for the Rad1/Rad10 endonuclease in recombination in plants. This is the first direct demonstration of the involvement of AtRad1p in homologous recombination in plants.