Murine spermatogonial stem cells: targeted transgene expression and purification in an active state | Zendy

Giuili Galicia | Zendy; Tomljenovic Andrea | Zendy; Labrecque Nathalie | Zendy; OuladAbdelghani Mustapha | Zendy; Rassoulzadegan Minoo | Zendy; Cuzin François | Zendy

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Murine spermatogonial stem cells: targeted transgene expression and purification in an active state

Author(s) -

Giuili Galicia,

Tomljenovic Andrea,

Labrecque Nathalie,

OuladAbdelghani Mustapha,

Rassoulzadegan Minoo,

Cuzin François

Publication year - 2002

Publication title -

embo reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 4.584

H-Index - 184

eISSN - 1469-3178

pISSN - 1469-221X

DOI - 10.1093/embo-reports/kvf149

Subject(s) - biology , stem cell , transgene , microbiology and biotechnology , cell sorting , germinal center , gene , flow cytometry , genetics , antibody , b cell

A 400 bp fragment of the spermatogonia‐specific Stra8 locus was sufficient to direct gene expression to the germinal stem cells in transgenic mice. A fractionation procedure was devised, based on immunomagnetic sorting of cells in which the promoter drives the expression of a surface functionally neutral protein tag. The purified cells expressed the known molecular markers of spermatogonia Rbm, cyclin A2 and EP‐Cam, and the β 1 ‐ and α 6 ‐integrins characteristic of the stem cell fraction. A 700‐fold enrichment in stem cells was determined by the ability of the purified fractions to re‐establish spermatogenesis in germ cell‐depleted recipient testes.

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