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STAG2 and Rad21 mammalian mitotic cohesins are implicated in meiosis
Author(s) -
Prieto Ignacio,
Pezzi Nieves,
Buesa José M,
Kremer Leonor,
Barthelemy Isabel,
Carreiro Candelas,
Roncal Fernando,
Martínez Alicia,
Gómez Lucio,
Fernández Raúl,
MartínezA Carlos,
Barbero José L
Publication year - 2002
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kvf108
Subject(s) - cohesin , establishment of sister chromatid cohesion , meiosis , biology , sister chromatids , microbiology and biotechnology , meiosis ii , chromosome segregation , mitosis , spindle apparatus , separase , genetics , chromosome , cell division , cell , gene
STAG/SA proteins are specific cohesin complex subunits that maintain sister chromatid cohesion in mitosis and meiosis. Two members of this family, STAG1/SA1 and STAG2/SA2, ‡ are classified as mitotic cohesins, as they are found in human somatic cells and in Xenopus laevis as components of the cohesin SA1 and cohesin SA2 complexes, in which the shared subunits are Rad21/SCC1, SMC1 and SMC3 proteins. A recently reported third family member, STAG3, is germinal cell‐specific and is a subunit of the meiotic cohesin complex. To date, the meiosis‐specific cohesin complex has been considered to be responsible for sister chromatid cohesion during meiosis. We studied replacement of the mitotic by the meiotic cohesin complex during mouse germinal cell maturation, and we show that mammalian STAG2 and Rad21 are also involved in several meiosis stages. Immunofluorescence results suggest that a cohesin complex containing Rad21 and STAG2 cooperates with a STAG3‐specific complex to maintain sister chromatid cohesion during the diplotene stage of meiosis.