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Distinct but overlapping domains of AKAP95 are implicated in chromosome condensation and condensin targeting
Author(s) -
Eide Turid,
Carlson Cathrine,
Taskén Kristin A,
Hirano Tatsuya,
Taskén Kjetil,
Collas Philippe
Publication year - 2002
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kvf089
Subject(s) - condensin , premature chromosome condensation , chromatin , biology , microbiology and biotechnology , prophase , chromosome , genetics , mitosis , chromosome segregation , gene , meiosis
A‐kinase (or PKA)‐anchoring protein AKAP95 is a zinc‐finger protein implicated in mitotic chromosome condensation by acting as a targeting molecule for the condensin complex. We have identified determinants of chromatin‐binding, condensin‐targeting and chromosome‐condensation activities of AKAP95. Binding of AKAP95 to chromatin is conferred by residues 387–450 and requires zinc finger ZF1. Residues 525–569 are essential for condensation of AKAP95‐free chromatin and condensin recruitment to chromosomes. Mutation of either zinc finger of AKAP95 abolishes condensation. However, ZF1 is dispensable for condensin targeting, whereas the C‐terminal ZF2 is required. AKAP95 interacts with Xenopus XCAP‐H condensin subunit in vitro and in vivo but not with the human hCAP‐D2 subunit. The data illustrate the involvement of overlapping, but distinct, domains of AKAP95 for condensin recruitment and chromosome condensation and argue for a key role of ZF1 in chromosome condensation and ZF2 in condensin targeting. Moreover, condensin recruitment to chromatin is not sufficient to promote condensation.