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The processivity factor β controls DNA polymerase IV traffic during spontaneous mutagenesis and translesion synthesis in vivo
Author(s) -
LenneSamuel Nathalie,
Wagner Jérôme,
Etienne Hélène,
Fuchs Robert P P
Publication year - 2002
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kvf007
Subject(s) - processivity , dna polymerase , dna polymerase ii , dna polymerase delta , dna clamp , biology , mutagenesis , polymerase , microbiology and biotechnology , dna polymerase i , dna polymerase mu , dna replication , dna , genetics , mutation , circular bacterial chromosome , gene , polymerase chain reaction , reverse transcriptase
The dinB ‐encoded DNA polymerase IV (Pol IV) belongs to the recently identified Y‐family of DNA polymerases. Like other members of this family, Pol IV is involved in translesion synthesis and mutagenesis. Here, we show that the C‐terminal five amino acids of Pol IV are essential in targeting it to the β‐clamp, the processivity factor of the replicative DNA polymerase (Pol III) of Escherichia coli . In vivo , the disruption of this interaction obliterates the function of Pol IV in both spontaneous and induced mutagenesis. These results point to the pivotal role of the processivity clamp during DNA polymerase trafficking in the vicinity of damaged‐template DNA.