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Tramtrack69 interacts with the dMi‐2 subunit of the Drosophila NuRD chromatin remodelling complex
Author(s) -
Murawsky Chris M,
Brehm Alexander,
Badenhorst Paul,
Lowe Nicholas,
Becker Peter B,
Travers Andrew A
Publication year - 2001
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kve252
Subject(s) - biology , chromatin , polytene chromosome , histone , nucleosome , microbiology and biotechnology , protein subunit , chromatin remodeling , acetylation , repressor , genetics , drosophila melanogaster , gene , transcription factor
dMi‐2, the ATPase subunit of the Drosophila nucleosome remodelling and histone deacetylation (dNuRD) complex, was identified in a two‐hybrid screen as an interacting partner of the transcriptional repressor, Tramtrack69 (Ttk69). A short region of Ttk69 is sufficient to mediate this interaction. Ttk69, but not the Ttk88 isoform, co‐purifies with the dNuRD complex isolated from embryo extracts. dMi‐2 and Ttk69 co‐immunoprecipitate from embryonic extracts, indicating that they can associate in vivo . Both dMi‐2 and Ttk69 co‐localize at a number of discrete sites on polytene chromosomes, showing that they bind common target loci. We also demonstrate that dMi‐2 and Ttk interact genetically, indicating a functional interaction in vivo . We propose that Ttk69 represses some target genes by remodelling chromatin structure through the recruitment of the dNuRD complex.