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The cyclin B1 gene is actively transcribed during mitosis in HeLa cells
Author(s) -
Sciortino Selvaggia,
Gurtner Aymone,
Manni Isabella,
Fontemaggi Giulia,
Dey Anup,
Sacchi Ada,
Ozato Keiko,
Piaggio Giulia
Publication year - 2001
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kve223
Subject(s) - mitosis , cyclin a , biology , cyclin a2 , cyclin b , cyclin d , chromatin , microbiology and biotechnology , cyclin b1 , transcription (linguistics) , cyclin , rna polymerase ii , promoter , gene , cell cycle , genetics , gene expression , cyclin dependent kinase 1 , linguistics , philosophy
In mammalian cells, the expression level of the cyclin B1 gene plays a critical role in the progression through mitosis. Here we demonstrate that the transcriptional activity of the human cyclin B1 promoter, as well as the rate of gene transcription, is high during mitosis. Indeed, the cyclin B1 promoter maintains an open chromatin configuration at the mitotic stage. Consistent with this, we show that the cyclin B1 promoter is occupied and bound to NF‐Y during mitosis in vivo . Our results provide the first example of RNA polymerase II‐dependent transcription during mitosis in mammalian cells.