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Impact of the six nucleotides downstream of the stop codon on translation termination
Author(s) -
Namy Olivier,
Hatin Isabelle,
Rousset JeanPierre
Publication year - 2001
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kve176
Subject(s) - stop codon , start codon , genetics , translation (biology) , biology , base pair , nucleotide , context (archaeology) , eukaryotic translation , open reading frame , computational biology , dna , gene , peptide sequence , messenger rna , paleontology
The efficiency of translation termination is influenced by local contexts surrounding stop codons. In Saccharomyces cerevisiae , upstream and downstream sequences act synergistically to influence the translation termination efficiency. By analysing derivatives of a leaky stop codon context, we initially demonstrated that at least six nucleotides after the stop codon are a key determinant of readthrough efficiency in S. cerevisiae . We then developed a combinatorial‐based strategy to identify poor 3′ termination contexts. By screening a degenerate oligonucleotide library, we identified a consensus sequence –CA(A/G)N(U/C/G)A–, which promotes >5% readthrough efficiency when located downstream of a UAG stop codon. Potential base pairing between this stimulatory motif and regions close to helix 18 and 44 of the 18S rRNA provides a model for the effect of the 3′ stop codon context on translation termination.