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A genetic look at the active site of RNA polymerase III
Author(s) -
Rozenfeld Sophie,
Thuriaux Pierre
Publication year - 2001
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kve136
Subject(s) - polymerase , active site , rna polymerase , biology , mutagenesis , rna , rna dependent rna polymerase , site directed mutagenesis , mutant , rna polymerase i , genetics , enzyme , microbiology and biotechnology , biochemistry , gene
rpc160‐112 , a mutant of the RNA polymerase III active site, is corrected in vivo by six second‐site mutants obtained by random mutagenesis. These mutants introduce single‐site amino acid replacements at the two large subunits of the enzyme. The mutated motifs are conserved in RNA polymerases I and II and, for some of them, in the bacterial enzyme, thus delineating key elements of the active site in eukaryotic RNA polymerases.