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BRCA1 can stimulate gene transcription by a unique mechanism
Author(s) -
Nadeau Guy,
Boufaied Nadia,
Moisan Annie,
Lemieux Karine M,
Cayanan Charmagne,
Monteiro Alvaro N A,
Gaudreau Luc
Publication year - 2000
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kvd059
Subject(s) - transcription (linguistics) , mechanism (biology) , gene , genetics , biology , transcription factor , microbiology and biotechnology , computational biology , philosophy , linguistics , epistemology
Most familial breast and ovarian cancers have been linked to mutations in the BRCA1 gene. BRCA1 has been shown to affect gene transcription but how it does so remains elusive. Here we show that BRCA1 can stimulate transcription without the requirement for a DNA‐tethering function in mammalian and yeast cells. Furthermore, the BRCA1 C‐terminal region can stimulate transcription of the p53‐responsive promoter, MDM2 . Unlike many enhancer‐specific activators, non‐tethered BRCA1 does not require a functional TATA element to stimulate transcription. Our results suggest that BRCA1 can enhance transcription by a function additional to recruiting the transcriptional machinery to a targeted gene.