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Prediction of structural domains of TAP reveals details of its interaction with p15 and nucleoporins
Author(s) -
Suyama Mikita,
Doerks Tobias,
Braun Isabelle C,
Sattler Michael,
Izaurralde Elisa,
Bork Peer
Publication year - 2000
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1093/embo-reports/kvd009
Subject(s) - nucleoporin , nuclear pore , nuclear transport , microbiology and biotechnology , biology , mutagenesis , nuclear export signal , biophysics , genetics , mutation , gene , cell nucleus , cytoplasm
Vertebrate TAP is a nuclear mRNA export factor homologous to yeast Mex67p. The middle domain of TAP binds directly to p15, a protein related to the nuclear transport factor 2 (NTF2), whereas its C‐terminal domain interacts with various nucleoporins, the components of the nuclear pore complex (NPC). Here, we report that the middle domain of TAP is also similar to NTF2, as well as to regions in Ras‐GAP SH3 domain binding protein (G3BP) and some plant protein kinases. Based on the known three‐dimensional structure of NTF2 homodimer, a heterodimerization model of TAP and p15 could be inferred. This model was confirmed by site‐directed mutagenesis of residues located at the dimer interface. Furthermore, the C‐terminus of TAP was found to contain a ubiquitin‐associated (UBA) domain. By site‐directed mutagenesis we show that a conserved loop in this domain plays an essential role in mediating TAP–nucleoporin interaction.